FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3953098
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG15650.017.8583851
TATACAG17850.015.6569555
CTAATAC15500.015.3595293
GCGCACT6000.014.94972311
GTATAGG14850.014.8704811
ACCCGCG5550.014.29739433
GTATATA23900.014.2925181
TATTAGG14900.013.8941182
TAGGTAG8800.013.72289855
CCTAGCG6350.013.58294130
TAATACT24300.013.4889394
TCTATAC17500.013.4069933
TATACTG21300.013.2829575
GTATAGA16750.013.183681
CTAGGAC5800.013.0874553
TATACTT25150.012.8958625
GTATAAG16150.012.8188831
TGTATAC20500.012.7914623
CATATAC19750.012.7531143
TCTAGAC12750.012.718863
Produced by FastQC (version 0.11.2)