FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4165790
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG10400.022.2300194
GCAGCGT12750.018.4042421
CAGCGTC14000.016.76022
CGTCAGA15450.014.9635375
AGCGTCA16050.014.4044973
ATAATAC22050.014.0842033
GTATAGA21950.013.9918351
GTATTAG14850.013.4778541
TATAATA23050.013.1737712
TATACTG21200.013.0209315
CTAGGAC6100.013.0105713
GTATATA28550.012.9329291
GTATTAT21300.012.6367151
TAATACT20650.012.5325554
GTATAAT22250.012.4073541
ACAGTGC17700.012.2793968
GTACTAG18450.011.9702381
GTATATG22250.011.9420791
GTATAAA20850.011.750911
GTCTTAT15300.011.7281931
Produced by FastQC (version 0.11.2)