FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences273305
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAG200.007353373351.7494511
TCGATAC500.00772517727.59970754
TATAATA658.631453E-426.5430362
TATACTG809.536399E-525.8747255
GTAGGAC700.001329911624.6471043
CTACACA700.001329911624.6471044
GTCTTAG700.001329911624.6471041
CTATACT902.1181206E-423.0039654
TGTACGA750.001989283122.99975612
GTATAAA1103.25085E-521.958331
GTGCCGA800.002890948221.5662171
TCGGTTG800.002894020421.56227144
TAATAGT1356.781953E-620.447974
GTCCTGA850.00410817620.2976171
TCTTATG850.00410817620.2976172
TTAGCCT1206.355884E-520.1284684
ACTCTCC1056.007324E-419.7140778
GTAACCA1056.007324E-419.7140779
GTTAATA900.00571777519.1699711
TGTACTC900.00571777519.1699713
Produced by FastQC (version 0.11.2)