FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences845048
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG2150.024.0750524
GTCTAGT2051.7334969E-920.1995561
TAATACT4300.020.0625444
ACAGTCC1551.0039876E-620.0319148
CCTAGAT1258.72281E-519.3242423
GCAGCGT3001.8189894E-1218.4040411
CTAATAC2701.5097612E-1017.8928183
TATCTCG3500.017.74255438
TCCTAGA2005.969978E-717.2537882
CGTATGC3600.017.24970443
ACCGTGC1602.9492856E-517.2497048
GTATATA4450.017.0599231
GTATTAT4250.017.0508041
TCTAAGC1855.432117E-616.787473
ATCCTAG1250.001937193216.5636371
CATATAC3601.8189894E-1216.2952443
TGTACAG2351.8110586E-716.1524835
TATAATA3950.015.7249712
ATACAGT3950.015.721256
GTCTAGG2654.1549356E-815.6260721
Produced by FastQC (version 0.11.2)