FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5161921
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGGC4850.014.93811123
GTATTAG18400.014.0661471
GTATATA36300.013.8796321
TATACAG24900.013.8571365
GTATAGA24100.013.6031131
TATACGG15100.013.48185
CTAATAC21850.013.1073913
CATATAC27500.013.0493933
TATACTG32600.012.912645
GTATAGG19250.012.9072431
TAGGTAG11500.012.9015965
CTATACT21250.012.8279664
CCTAATA20250.012.7798542
TAGGACA15100.012.1112394
TACACAG20250.012.0977935
GCGCACT7450.012.04000911
CTACACA15950.011.8984844
TAATACT33750.011.6552274
CGCGCAT7500.011.49986340
ACCGCGG5107.185008E-1011.49975118
Produced by FastQC (version 0.11.2)