FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3442590
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG12950.020.2506331
TAGGTAG8450.018.3746515
GTATAGA18200.015.7362591
CTAATAC15500.015.1372423
GTATATA26950.014.9803471
CTAGGAC6050.014.828173
TCTATAC13800.014.5016843
AGCGGGG5250.014.45693350
CCTAGCG6450.014.4420730
TATACAG17950.014.4165075
TAGACAG12300.014.3063335
CGCACCT7000.014.2926566
CCGCACC8150.013.96912165
TATTAGG14850.013.9410132
CTATACT16450.013.6336054
TCCGCAC7850.013.62400564
TATACCG11000.013.4877365
TAATACT24450.013.4062714
TTAGGTA12100.013.4021894
CCTAATA14700.013.3791062
Produced by FastQC (version 0.11.2)