FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2900138
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG10100.018.4499511
TCCGCAC6100.018.09800364
CCGCACC6350.016.84218865
CTAATAC12400.016.6963863
CGCACCT6200.016.69321866
GTATATA16750.016.275531
TATTAGG10500.016.103292
TAATACT18700.016.0529774
GCGTCAG6400.015.6349294
GTATAGA12800.015.3669521
CCTAATA12300.015.1494382
TACTATG13250.015.1015887
ATACTAT16550.014.8005686
GTCCTAG4152.1827873E-1114.1359071
TATACAG16050.013.7588725
CTATGCA11850.013.6832749
ACCGTGT4557.2759576E-1213.6480838
TATACTG16900.013.4751975
TATACCG8750.013.407535
ACTATGC14700.013.3772868
Produced by FastQC (version 0.11.2)