FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5503713
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC12950.020.24666264
GTATTAG24650.019.4588851
CGCACCT13550.018.84091666
TAGGTAG13650.018.1998845
CCGCACC13900.018.1183165
TATTAGG21850.017.6871992
ACTATGC28200.016.393268
GTATAGA29000.016.1830751
CTAATAC26450.016.0460433
CCTAATA26300.015.7441032
TAATACT39700.015.7314484
TATACAG31900.015.6836145
CTATGCA24350.015.1598329
TCTATAC25650.014.6631623
TACACCG11550.014.6380395
CCTAGCG10700.014.50968730
GTATATA47700.014.3964291
TATACTG38350.014.21544555
TAGTCAG15550.013.9771879
GGTAGTC16050.013.9716587
Produced by FastQC (version 0.11.2)