FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5752232
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT12750.025.16445266
TCCGCAC12950.022.91096764
CCGCACC14050.022.34496965
CTAATAC27950.021.7263683
TAGGTAG13550.018.8424475
CCTAATA25750.018.222762
TAATACT40950.017.946594
GTATTAG24450.017.7818981
ACTATGC29050.016.3889628
GTATAGA28050.016.1147981
CTATGCA25300.016.0908879
TATACAG31350.015.5176695
ACCTAAT29800.015.2842481
ATACTAT38400.014.91404156
AATACTA38850.014.7421885
TACTATG30250.014.4842777
TATTAGG22550.014.3824812
GTAGGAC9850.014.3616353
TTAGGTA20050.014.2830174
ATCAGCC22250.013.79986154
Produced by FastQC (version 0.11.2)