FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2284229
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG9750.022.2973251
CGCACCT4600.021.74965566
TAGACAG8350.018.5945555
CTAATAC10750.017.9745643
GTATAGG8450.017.9685381
TAATACT16500.017.774784
GTATAGA10500.017.4182031
CCGCACC5600.017.24972765
TAGGTAG6500.016.9862065
TCCGCAC5300.016.92426164
CCTAGCG4700.016.8834530
TATTAGG9200.016.877322
GCGTCAG4950.016.7292064
TCTATAC11000.015.9976523
GCGACTC5150.015.40752334
TATACTG15750.015.3347695
CCTAATA10450.015.1886892
TTAGGTA8550.014.9315494
TAGTCAG6050.014.8262119
GACTAGG5400.014.6942128
Produced by FastQC (version 0.11.2)