FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3330575
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG8450.019.5997565
GTATTAG13800.017.7540571
GCGTCAG7450.017.5994874
CTAATAC14100.016.8853153
ACCGCGC2907.543349E-915.4653368
TATACAG17300.015.3571565
CGCACCT7400.014.91874366
GTACTAG11900.014.7890931
GTATAGA14550.014.7043911
TAATACT23950.014.5508564
TAGTCAG9250.013.7998389
TATTAGG14100.013.4595112
TCCGCAC7200.013.41650964
GTATATA27650.013.3538651
ACAGTGC9400.013.212618
TCTATAC15600.013.0498833
TTAGGTA13300.012.9715254
TAGGCTA4601.2732926E-1012.7513815
ACTGTGC8950.012.720528
CACTGTG10750.012.5161317
Produced by FastQC (version 0.11.2)