FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5015925
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCACC9850.017.1620365
GTATTAG23550.016.8514371
TCCGCAC9650.016.80271164
GCGTCAG11500.016.50294
CTAATAC24950.015.9050733
TAGGTAG13150.015.4810355
CGCACCT10050.015.1041266
TATTAGG24400.013.8595462
TATACGG18850.013.5454715
GGTAGTC15050.013.2953667
GCAGCGT15250.013.1246631
TATACAG31750.012.9323375
TAATACT41600.012.8568264
TATACCG15600.012.8285375
CAGCGTC15350.012.8138382
TAGTCAG13200.012.8065159
GTATATA62200.012.7604931
CATATAC35600.012.4070453
ACATAGG13400.012.35853231
TTAGGTA21200.012.2073344
Produced by FastQC (version 0.11.2)