FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4400574
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG20000.018.115391
TAGGTAG10400.017.5833385
GCGTCAG9600.016.1735364
TCCGCAC8450.015.10634564
CCGCACC9150.015.08180265
TATACAG30700.014.947625
TATACTG30550.013.8916135
GTATAGA23150.013.8618221
GTATATA49900.013.4841161
TATTAGG21700.013.197062
CTAATAC21550.013.1288113
GTGTAAG10000.013.1120921
CGCACCT9750.013.09216666
CTGCGCG2651.1161146E-513.0188759
TATACGG16500.012.9648215
TTAGGTA17550.012.7790914
TCTATAC21450.012.5466013
TAGTCAG11850.012.5189939
CAGCGTC12750.012.4481962
GCAGCGT13100.012.1164381
Produced by FastQC (version 0.11.2)