FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3847872
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG17200.017.454841
CTAATAC18650.016.4663243
CCGCACC7150.016.40511965
GCGTCAG8650.016.3548894
TCCGCAC7150.015.44011264
TAGGTAG8950.015.0352335
TATACAG26400.014.8994255
GTATAGA20300.014.4493361
CGCACCT8350.014.04749766
CCTAGCG4609.094947E-1213.5003130
TATTAGG17500.013.4077642
GCAGCGT10300.013.4013271
TATACTG25900.013.1887625
CGACGGG3457.4005584E-812.99962349
GTATATA43550.012.6781781
TATACGG13600.012.4315635
TAATACT33000.011.9198494
CAGCGTC11700.011.7966752
GTCCTAG5301.09139364E-1011.719841
ACTATGC18150.011.5948568
Produced by FastQC (version 0.11.2)