FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5785422
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC12050.022.3316764
CGCACCT13350.019.89862666
CCGCACC13650.019.71403765
CTAATAC29050.018.1723673
GTATTAG26550.017.9353891
TAGGTAG14900.016.2092515
GTATAGA28700.015.5097241
CCTAGCG7800.015.48097730
TAATACT50750.014.549114
GTATATA69800.014.5341011
CCTAATA35700.013.9174772
ACTATGC30800.013.7774248
GTCTAGG12050.013.7451791
CTATGCA28450.013.58155259
TAGTCAG14250.013.5577259
GACTAGG9700.013.5152958
TCACACA21200.013.34451930
TACTATG35150.013.2501917
ATCAGCC20750.013.13477554
TATACAG41050.013.111815
Produced by FastQC (version 0.11.2)