FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3857530
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG7750.020.4787854
TAGGTAG9900.017.0766755
CCGCACC7700.017.02556665
CTAATAC19900.016.6447563
CGCACCT7700.016.57752466
TCCGCAC7650.016.23490364
GTATTAG16850.015.5628781
TATACAG22850.015.2502735
TAGTCAG9900.014.6362029
GCAGCGT10700.014.5112541
TATACTG24800.014.0511595
CAGCGTC11300.014.0457312
TACACCG8700.013.8800565
TATACGG13800.013.0006985
ACTATGC18100.012.9611828
TATTAGG18000.012.8430232
CCTATAC12100.012.8317673
GTATATA41450.012.8195391
CATATAC25300.012.6829933
GGTAGTC10700.012.5746277
Produced by FastQC (version 0.11.2)