FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5335211
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT10950.020.47920266
TCCGCAC11350.020.3653964
GCGTCAG12050.020.0431294
TAGGTAG14550.019.6820165
CCGCACC12750.017.85860665
GTATTAG25300.017.5941561
GCAGCGT14050.017.4373821
TATTAGG24550.016.7250042
CTAATAC26350.016.6299083
TAGTCAG14350.016.3482329
GGTAGTC16200.016.1849867
CCTAATA26150.014.9099972
CAGCGTC16200.014.9091812
ACTATGC27300.014.5327898
CTATGCA21250.014.4492359
TAATACT40550.014.3797174
ATTAGGT25850.014.0150763
CCTAGCG9150.013.95091130
TATACGG18800.013.9479465
ACATAGG16950.013.84079831
Produced by FastQC (version 0.11.2)