FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4186472
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG10300.019.0949134
GTATTAG16200.018.959761
CTAATAC19750.017.1220053
GCAGCGT12400.016.1422541
CCGCACC9000.015.71624465
GTATAGA21450.015.6063851
CGCACCT9050.015.24820966
CGCGCAT3959.094947E-1214.8478840
CAGCGTC13700.014.6087622
TCCGCAC9050.014.48579964
TAATACT31700.013.8237084
TATACTG26250.013.5388815
TAGGTAG10000.013.1116855
TATTAGG16900.012.8635212
AGCGTCA15400.012.7717323
GCGACTC6550.012.641335534
TATACCG13650.012.6389875
TATATAC45500.012.5890313
TATACGG15850.012.4085345
TTAGGAC9850.012.26104453
Produced by FastQC (version 0.11.2)