FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454841
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG10670.23458747122620874RNA PCR Primer, Index 17 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG6100.13411279985753263RNA PCR Primer, Index 17 (96% over 28bp)
TCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG5010.11014838152233418TruSeq Adapter, Index 12 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG803.45608E-1143.12204469
AAGGGGG3650.037.8056369
GGTAGTC953.195455E-729.0538377
GCCGTCT6450.027.81204846
ATGCCGT7350.027.22262644
TGCCGTC7300.026.93651245
ATCTCGT6950.026.31044237
AAAGGGG5250.026.28391568
ACAGCGT1208.4910425E-825.876078
CCGTCTT6950.025.8111847
GTATGCC7850.025.48869742
CGTATGC7900.025.3273841
AATCTCG7300.025.04898536
TATGCCG8150.024.97374743
TCGTATG8450.024.90635940
CTGCTTG6950.024.81844354
TATACCG1401.3777026E-824.652015
TCTCGTA6850.024.17618438
GCCGTCG1001.5617929E-524.14834447
CTCGTAT6950.023.82832539
Produced by FastQC (version 0.11.2)