FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4974265
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG23350.017.5848771
TAGGTAG13850.016.9397815
TCCGCAC9650.016.08788564
GCGTCAG10200.014.8833684
TATTAGG25100.014.708472
CGCACCT10600.014.64604766
GGTAGTC14400.014.3748237
GTATAGA26450.014.3498141
CCGCACC11100.014.29712265
CTAATAC27000.013.8012183
CCTAGCG6650.013.48896330
TAGTCAG14350.013.2228349
TATACAG35800.013.0106635
TAGACTG14000.012.8151595
GTATATA64000.012.3462421
TATACCG16400.012.2020525
TAATACT42850.012.0778364
ACTATGC23500.011.597738
CTTAGGG6251.8189894E-1211.5930233
GCGACTC7450.011.57715634
Produced by FastQC (version 0.11.2)