FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4082580
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT7750.016.91587866
CCGCACC7400.015.85111565
GTATTAG18450.015.7101621
TCCGCAC8100.014.48126664
TAGGTAG10850.014.3106465
TATACGG16500.014.0109415
CTAATAC19950.013.8364133
ACCGCGC2506.1173087E-613.7999648
GTATAGA21350.013.4146081
CCTAGCG5500.013.17269230
GTATATA51500.012.6634571
CATATAC29100.012.5686693
GCGACTC5359.094947E-1212.25215334
GACTAGG5701.8189894E-1212.1052318
CCGGCCC4601.7425918E-911.99982213
TAATACT36300.011.881884
TAGACAG13100.011.852715
ACAGTGC10800.011.8194138
TATACTG29200.011.69846255
GGCCCGC4752.8849172E-911.6208815
Produced by FastQC (version 0.11.2)