FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5010656
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG13450.017.187875
GTATTAG23450.015.8923991
TCCGCAC9200.015.3747964
CCGCACC10100.014.68791965
TAGTCAG13700.014.1019989
GTATAGA25350.013.7483931
GTATATA69100.013.7329291
TATTAGG26850.013.6222382
GGTAGTC13750.013.5489077
TATACTG37750.013.0703925
CGCACCT10650.012.9575766
TACACCG9600.012.9390035
ACATAGG14300.012.78666831
CTAATAC25950.012.76499753
TAGACCG13550.012.4774655
TTAGGTA21750.012.2152114
TCTATAC24450.012.1368643
GGCGTCG6300.012.04757632
TATACCG16400.011.9922485
TATACAG37000.011.9365215
Produced by FastQC (version 0.11.2)