FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4186042
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC2753.7362042E-916.3088198
TAGGTAG10500.015.1158415
TCCGCAC8300.014.96360764
GTATTAG19900.014.5648331
CCGCACC8550.014.52607465
CGCACCT7900.014.41115366
GTATATA56250.013.8632561
CCTACAC7250.013.8015833
CCTAGCG5550.013.67610230
TATACAG32750.013.5907115
CGCGGCC2304.276455E-513.49977613
TATACGG17150.013.479525
TACACCG10500.013.4728155
ATACCGT17150.013.2769056
TAGACAG14300.013.2705965
GTATAGA24500.013.2385711
CATATAC33900.013.0280433
TATACCG12600.012.8703725
CGCGCAC2952.376557E-612.86419310
CATACAC15600.012.8283953
Produced by FastQC (version 0.11.2)