FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3677383
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC6250.018.2155464
CCGGCCC4200.018.07097213
CCGCACC6550.017.3812465
GTATTAG17600.017.0591141
GTCCGGC4750.015.97854411
TATACTG24150.015.0022745
CGGCCCG4151.8189894E-1214.96347914
TAGGTAG9450.014.6053885
CCGTGCG1901.2831188E-414.52594959
AGTCCGG7450.013.89226610
TATACAG25050.013.6367975
TCTATAC18000.013.61113553
TCCGGCC5900.013.44881312
AGACCGC3608.923962E-913.4168756
GCCCGCC4508.913048E-1113.03300416
CGCACCT7950.013.01853966
ATACGGT16000.012.9377016
TATACGG14700.012.910125
TAGTCAG8600.012.8368859
GTATATA49450.012.7014991
Produced by FastQC (version 0.11.2)