FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4316229
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCACC9350.020.29387965
TCCGCAC8500.019.88864
TAGGTAG13250.019.2688145
TAGTCAG10500.018.728359
CGCACCT9800.017.9538766
GTATTAG22100.017.7984391
GGTAGTC13600.017.2497967
CTAATAC22750.016.8339393
GCGTCAG7950.015.6233634
TATTAGG22550.015.3002192
TTAGGTA18650.014.4296214
ACATAGG13900.014.1474831
GTATAGA22100.014.0513991
ACTATGC20350.013.9015568
GTATATA53150.013.8924831
TATATAC60400.013.7094013
CTATGCA18450.013.6502469
TATACAG30450.013.5966865
ATAGGAG14100.013.45744433
TACTATG25100.013.0576157
Produced by FastQC (version 0.11.2)