FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4193674
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC7100.018.46448964
TAGGTAG12300.016.5510775
GCGTCAG7850.016.2634034
GTATTAG18550.015.9981651
TAGTCAG11100.015.5402899
CTAATAC19400.015.4737973
CCGCACC8500.015.4232865
TATACCG12500.014.630035
CGCACCT9300.014.46750766
GTCCTAG6000.014.3781951
TTAGGTA17200.013.6414274
TATACAG28450.013.5836075
ACATAGG11900.013.04600631
GTATAGA19650.012.99525451
TATTAGG22700.012.9206042
GCCGTCT12100.012.8303746
AGCGGGG4601.2914825E-1012.74979350
CGGCCCG5700.012.7103214
TAATACT35550.012.6177854
ACTATGC21900.012.44500358
Produced by FastQC (version 0.11.2)