FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2579581
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC15600.026.75904345
GCCGTCT15500.026.48653246
GATCTCG15700.025.49039636
GTATGCC17350.024.45769142
TCTCGTA17000.024.35289238
CTCGTAT17350.024.0604739
CGTATGC18000.023.57495341
TATGCCG18050.023.50919343
ATCTCGT17700.022.99995437
TCGTATG18900.022.99995440
CCGTCTT18400.022.49952147
ATGCCGT19250.022.22290244
AGATCTC18700.021.5855235
CTAGATC19250.020.4311333
CTGCTTG19250.020.43073354
AAGGGGG11350.019.1493769
ACTAGAT23050.018.26026732
CGTCTTC23650.017.94253348
TCCGCAC5000.017.93961764
TAGGTAG7350.017.8394665
Produced by FastQC (version 0.11.2)