FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3060203
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG8300.017.8752355
AAGGGGG8950.017.7315469
TAGTCAG7550.016.9069969
GGTAGTC9550.016.6175177
GCGAATC19950.016.25536233
AATCGCG6800.015.22032836
TATTAGG14350.014.9083482
GTATTAG15250.014.7080321
TATACAG19950.014.527735
CTATACT15050.014.44397454
ACATAGG9500.013.7999931
TATACGG11150.013.6156755
AAAGGGG14750.013.56586969
CTAATAC13900.013.4050583
GGCGAAT25050.013.35927232
GCGTCAG6200.013.3567934
TCCGCAC6850.013.094666564
CACGCAG5950.012.75608516
ACGGTGT8950.012.7204538
ATAGGAG9500.012.71030933
Produced by FastQC (version 0.11.2)