FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4785688
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG19950.019.025821
TAGGTAG13550.016.0418345
GCGTCAG9650.015.0168664
GCTCGCG4900.014.081461
TATTAGG23150.014.0103112
GCGAATC15450.013.84457633
TACACCG8500.013.8010695
GGTAGTC13450.013.5945717
TATACAG32750.013.5903665
TATACGG18950.013.473345
GTATATA59300.013.4415981
TCCGCAC9250.013.426805564
TAGTCAG13450.013.338079
GCAGCGT11350.013.0727271
CCTACAC7450.012.967723
TTAGGTA21100.012.9181984
CTAGTAC14300.012.78797153
CTTACAC13050.012.6909053
GTATAGA20750.012.6383131
CATATAC36650.012.6151573
Produced by FastQC (version 0.11.2)