FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3704903
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG8700.021.0212735
GTATTAG15600.017.918351
GCGAATC20250.017.20745733
TAGTCAG8750.016.1655439
GGTAGTC10950.014.4929977
GGCGAAT26050.014.30330532
CATATAC23050.014.07249453
TAATACT27150.013.5994784
AAGGGGG13050.013.48243469
TATTAGG18000.013.4196032
CTAATAC16250.013.3783123
GCGTCAG7000.013.3098764
TTAGGTA14150.013.1687824
TATACAG21500.012.8396735
AGGCGAA30050.012.62898931
ACTATGC15950.012.3290238
CGAATCG9800.012.32129834
CGAATCT20850.012.24447534
ACATAGG10200.012.17650731
TATAATA33850.012.1311632
Produced by FastQC (version 0.11.2)