FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3747861
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG12700.021.1885369
GCGAATC22500.020.54705633
GCGTCAG8350.018.1819254
GGCGAAT27300.017.56626732
GTATTAG16400.016.8322811
TAGGTAG10000.016.2168225
CCGCACC8050.015.8568165
TCCGCAC7900.015.72118864
CGCACCT8200.015.56674466
CTAATAC19150.015.4952143
AGGCGAA31550.015.30932331
AAAGGGG18400.014.99968468
GCAGCGT10500.014.7883621
CGAATCG12600.014.78579734
CAGCGTC11100.014.6099442
AAGGCGA33400.014.56464830
TATACGG12900.014.4434845
TATACAG22750.014.2565465
CGAATCT21150.013.539083534
TTAGGTA14550.013.5171634
Produced by FastQC (version 0.11.2)