FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3017462
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAC13600.016.9985983
GTATTAG13650.015.6737251
TAGGTAG7500.015.6418345
GCGAATC14850.015.10102933
TAGTCAG7050.014.6806279
TATTAGG14400.014.3771622
TATACTG23000.014.251675
GTATAGA13450.013.8543021
GTATATA36800.013.4091561
TATACGG12200.013.29254155
CACGCAG6800.013.19097416
CGCGGCC2102.9981203E-413.14265413
ATACGGT10800.013.0970216
TACGGTG9750.013.0921077
TATACAG20450.012.8230195
ATTATAC22050.012.6751643
ACGGTGT9800.012.6732758
ACATAGG7700.012.54546931
CATATAC23850.012.4419163
TATTATA36450.012.3063762
Produced by FastQC (version 0.11.2)