FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1605986
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG3800.027.241554
GCAGCGT4650.023.0046921
TAGGCCG1108.229104E-418.8214345
GCGCGTC1501.6948034E-518.39974442
CAGCGTC5650.018.321752
TAGTCAG3650.017.0134629
CGCGTCA1503.526594E-416.09977543
CCTAGCG2151.2761266E-616.04628830
TATACGG5750.015.6026965
AGCGTCA6800.015.2232193
GACGCGC1400.00413217314.78550738
CGTCAGA7050.014.6833895
TAGGTAG4950.014.6388935
CCTAGGG1450.00521010114.278332
ATTATAC10200.014.2083383
CGATCAC2708.7672925E-714.05535924
ATGTGTA8400.013.9640911
GCGACTC2751.0782278E-613.79980834
GATGTGT8000.013.79980810
CTGTGCG2001.9824642E-413.7998089
Produced by FastQC (version 0.11.2)