FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1186235
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG4900.027.45861469
GCGAATC10500.024.31378233
GCGTCAG2600.022.5619814
GGCGAAT11600.022.30557332
AATCGCG2655.456968E-1219.52789536
CCTGGAC1952.1498636E-819.4652393
AGGCGAA13700.019.39092431
TCTCGTA6300.019.1662738
TGCCGGC1801.9818981E-719.1662745
AAAGGGG7550.018.7347168
CGTATGC5900.018.71147541
ATCTCGT6850.018.6346537
CGAATCT9650.018.59028634
GTATGCC6000.018.39961842
AATCTCG8550.018.15751636
GCAGCGT3250.018.0495831
AAGGCGA15350.017.9808430
CTCGTAT6950.017.87013439
CGAATCG4700.017.61665534
TGCCGTC4900.017.60167545
Produced by FastQC (version 0.11.2)