FastQCFastQC Report
Fri 28 Oct 2016
HG7CVAFXX_n01_mini02_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7CVAFXX_n01_mini02_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1741514
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG6400.024.79653469
GCGAATC13600.023.08424233
CGAATCG6150.021.3167834
GTATTAG8750.020.8998551
TAGGTAG5300.020.1812845
AATCGCG4050.019.59232336
TCCGCAC2951.8189894E-1218.71160764
GGCGAAT17450.018.58427232
TGCCGTC6400.018.32787345
GGTAGTC4750.018.1581697
GCGACTC2501.0513759E-917.93975434
ATCGCGG1954.5970955E-717.69206637
AAAGGGG9000.017.63309368
GCGTCAG3153.6379788E-1217.5255834
CCTAGCG2601.7698767E-917.2502630
GCCGTCT6100.016.9669846
TAGTCAG5150.016.7473439
TATGCCG7350.016.42834543
GTCCCGA1503.526801E-416.0997811
ATGCCGT6900.015.99978144
Produced by FastQC (version 0.11.2)