FastQCFastQC Report
Thu 6 Dec 2018
HG77GBGX9_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG77GBGX9_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21351560
Sequences flagged as poor quality0
Sequence length99
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3243361.519027181152103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG412600.073.536752
AAGCAGT525350.058.151921
GTATCAA605050.050.2280969
GCAGTGG611550.050.1510243
AGTGGTA646100.047.6475455
CAGTGGT648000.047.3853954
GGTATCA666200.045.771998
GTGGTAT698500.044.1199956
TGGTATC805600.038.213947
ATCAACG567200.030.0376510-11
AGTACAT574350.029.68635620-21
AGAGTAC587100.029.16065818-19
TACATGG582750.028.99585322-23
CATGGGG329750.028.94827324-25
CGCAGAG572550.026.95313516-17
GTACATG568200.026.71051222-23
CAACGCA642100.026.58044812-13
CAGAGTA579500.026.56230718-19
GAGTACA587250.026.0888520-21
GCAGAGT663800.026.01127416-17
Produced by FastQC (version 0.11.5)