FastQCFastQC Report
Thu 6 Dec 2018
HG77GBGX9_n02_10x_cut_dec18_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG77GBGX9_n02_10x_cut_dec18_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77031981
Sequences flagged as poor quality0
Sequence length99
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4479780.5815480715730262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG2389650.068.1856462
AAGCAGT2489600.065.6876451
GCAGTGG2806400.057.9744383
AGTGGTA2958300.055.0541235
CAGTGGT3147450.051.7710884
GGTATCA3445200.047.2230078
GTGGTAT3466150.047.0356186
TGGTATC3558800.045.7643287
GTATCAA3724700.043.7067579
ACGCAGA3353050.027.26255814-15
CAACGCA3388850.026.98277312-13
ATCAACG3471050.026.4233710-11
GCAGAGT3494450.026.13776216-17
AGTACAT3469050.026.08514620-21
AGAGTAC3537700.025.78280418-19
TACATGG3516200.025.66645622-23
CATGGGG2049700.025.01666824-25
GTACATG3367850.023.90543222-23
TCAACGC3445450.023.63004912-13
CGCAGAG3490400.023.41169516-17
Produced by FastQC (version 0.11.5)