FastQCFastQC Report
Thu 6 Dec 2018
HG77GBGX9_n02_10x_cut_dec18_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG77GBGX9_n02_10x_cut_dec18_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59752709
Sequences flagged as poor quality0
Sequence length99
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3427520.5736175074505827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG1844250.068.4805152
AAGCAGT1930100.065.7021
GCAGTGG2170350.058.138013
AGTGGTA2280000.055.346665
CAGTGGT2413200.052.352064
GGTATCA2653750.047.5562258
GTGGTAT2675250.047.2034956
TGGTATC2748250.045.9587677
GTATCAA2875750.043.891529
ACGCAGA2573100.027.39219314-15
CAACGCA2595850.027.14047812-13
ATCAACG2660800.026.54194610-11
GCAGAGT2687450.026.20539716-17
AGTACAT2664550.026.20376820-21
AGAGTAC2708450.025.94334818-19
TACATGG2696400.025.76702722-23
CATGGGG1560150.025.20429824-25
GTACATG2584750.024.14877322-23
TCAACGC2645400.023.85094612-13
CGCAGAG2681350.023.6162216-17
Produced by FastQC (version 0.11.5)