FastQCFastQC Report
Thu 6 Dec 2018
HG77GBGX9_n02_10x_cut_dec18_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG77GBGX9_n02_10x_cut_dec18_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53674591
Sequences flagged as poor quality0
Sequence length99
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3164490.5895694668637531No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG1670700.068.382972
AAGCAGT1744350.065.732921
GCAGTGG1967100.058.029813
AGTGGTA2064050.055.336215
CAGTGGT2189600.052.2256554
GGTATCA2405250.047.486658
GTGGTAT2410550.047.419276
TGGTATC2485050.046.0300757
GTATCAA2610550.043.7515039
ACGCAGA2335200.027.45200514-15
CAACGCA2358800.027.20845812-13
ATCAACG2410850.026.66996210-11
GCAGAGT2439650.026.26162316-17
AGTACAT2423900.026.24442320-21
AGAGTAC2460000.026.03730818-19
TACATGG2453500.025.83076122-23
CATGGGG1433500.025.11628524-25
GTACATG2359200.023.9802922-23
TCAACGC2395050.023.85581212-13
CGCAGAG2425300.023.65352816-17
Produced by FastQC (version 0.11.5)