FastQCFastQC Report
Thu 6 Dec 2018
HG77GBGX9_n02_10x_cut_dec18_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG77GBGX9_n02_10x_cut_dec18_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53881606
Sequences flagged as poor quality0
Sequence length99
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3130160.58093294398092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG1633300.068.238322
AAGCAGT1716650.065.152391
GCAGTGG1925750.057.7336163
AGTGGTA2039600.054.6346785
CAGTGGT2152000.051.8013884
GGTATCA2377750.046.8474278
GTGGTAT2393650.046.5873836
TGGTATC2473400.045.0858887
GTATCAA2587750.043.080789
ACGCAGA2296850.027.09213314-15
CAACGCA2334150.026.69167712-13
ATCAACG2388700.026.1687410-11
GCAGAGT2404050.025.86895216-17
AGTACAT2393750.025.78820820-21
AGAGTAC2429350.025.56087118-19
TACATGG2422950.025.37127722-23
CATGGGG1404950.024.76275324-25
GTACATG2325250.023.69126922-23
TCAACGC2368400.023.52911812-13
CGCAGAG2391850.023.35433416-17
Produced by FastQC (version 0.11.5)