FastQCFastQC Report
Thu 6 Dec 2018
HG77GBGX9_n02_10x_ctr2_dec18_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG77GBGX9_n02_10x_ctr2_dec18_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44138178
Sequences flagged as poor quality0
Sequence length99
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3347570.7584295844744656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT4273250.084.295861
AGCAGTG4548450.079.036282
GCAGTGG5523450.065.013943
CAGTGGT5647750.063.5990224
AGTGGTA5772350.062.333495
GTGGTAT6084100.059.1189276
GGTATCA6389500.056.2199828
TGGTATC6408250.056.071037
GTATCAA6504050.055.238089
ACGCAGA6097300.032.831914-15
ATCAACG6149850.032.5392110-11
CAACGCA6152400.032.4986512-13
GCAGAGT6179800.032.3609316-17
AGTACAT6231000.032.01114320-21
TACATGG6358550.031.254822-23
AGAGTAC6398650.031.25443318-19
CATGGGG3490600.031.19654724-25
TCAACGC6105300.029.43123812-13
CGCAGAG6135650.029.3694716-17
AACGCAG6177950.029.17019514-15
Produced by FastQC (version 0.11.5)