FastQCFastQC Report
Thu 6 Dec 2018
HG77GBGX9_n02_10x_ctr2_dec18_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG77GBGX9_n02_10x_ctr2_dec18_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66107215
Sequences flagged as poor quality0
Sequence length99
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5398840.816679389685377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT6416950.084.2243351
AGCAGTG6830950.078.9774252
GCAGTGG8336750.064.668163
CAGTGGT8512300.063.313324
AGTGGTA8701400.061.9976125
GTGGTAT9174050.058.7801746
GGTATCA9633000.055.9239858
TGGTATC9658000.055.7866677
GTATCAA9794800.054.983769
ACGCAGA9199150.032.59058814-15
ATCAACG9275650.032.3089210-11
CAACGCA9285350.032.25153712-13
GCAGAGT9343350.032.10304316-17
AGTACAT9404150.031.7700620-21
AGAGTAC9670950.031.0096218-19
CATGGGG5265800.030.96851724-25
TACATGG9616200.030.95978422-23
TCAACGC9212050.029.2390712-13
CGCAGAG9270550.029.16131616-17
AACGCAG9316550.028.96967914-15
Produced by FastQC (version 0.11.5)