FastQCFastQC Report
Thu 6 Dec 2018
HG77GBGX9_n02_10x_ctr2_dec18_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG77GBGX9_n02_10x_ctr2_dec18_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37989980
Sequences flagged as poor quality0
Sequence length99
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2962130.7797134928736472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3705250.084.223341
AGCAGTG3938600.079.065412
GCAGTGG4801150.064.8325043
CAGTGGT4903950.063.4283334
AGTGGTA4998300.062.32015
GTGGTAT5268600.059.109576
GGTATCA5524700.056.3068778
TGGTATC5546900.056.0820167
GTATCAA5622200.055.3318569
ACGCAGA5282350.032.7695314-15
ATCAACG5330350.032.4729910-11
CAACGCA5331400.032.4385712-13
GCAGAGT5368650.032.22883616-17
AGTACAT5398950.031.92226820-21
AGAGTAC5546200.031.19085918-19
TACATGG5518050.031.11980822-23
CATGGGG3025900.031.00986924-25
TCAACGC5289150.029.42629212-13
CGCAGAG5321300.029.33843216-17
AACGCAG5352000.029.1372314-15
Produced by FastQC (version 0.11.5)