FastQCFastQC Report
Thu 6 Dec 2018
HG77GBGX9_n02_10x_ctr2_dec18_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG77GBGX9_n02_10x_ctr2_dec18_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41239912
Sequences flagged as poor quality0
Sequence length99
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3271990.7934037298624692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT4009050.084.372471
AGCAGTG4266650.079.1190952
GCAGTGG5201250.064.87023
CAGTGGT5317450.063.4369854
AGTGGTA5426100.062.2220045
GTGGTAT5716800.059.0429156
GGTATCA6001650.056.187198
TGGTATC6021900.055.9855847
GTATCAA6101450.055.2779439
ACGCAGA5727400.032.7834514-15
ATCAACG5771000.032.52822510-11
CAACGCA5774950.032.47211512-13
GCAGAGT5812200.032.29068816-17
AGTACAT5850050.031.94401720-21
AGAGTAC6014550.031.18409218-19
TACATGG5989550.031.09634622-23
CATGGGG3284550.031.05338924-25
TCAACGC5725850.029.48217212-13
CGCAGAG5766300.029.34743516-17
AACGCAG5805050.029.13519314-15
Produced by FastQC (version 0.11.5)