FastQCFastQC Report
Thu 6 Dec 2018
HG77GBGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG77GBGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21351560
Sequences flagged as poor quality0
Sequence length27
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGC363380.17018896979892803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGC38250.019.89712521
ACGAGCC57550.019.2380121
CGAGCCG28950.018.7205182
GCATGCG43000.018.5451451
GTAACTC46450.018.4916843
GCGCGAT25500.018.4658851
TGAGAGG44350.018.4319341
CTCCGTA51000.018.3005077
CTAGATG21150.018.2673326
ATGCGAG20600.018.1974243
TCCGTAG48700.018.1948348
GACCGGA15550.018.1617019
GCCTGTG33200.018.08808510
CCGTAGT44700.018.0379969
GCGGGTG15150.018.0250912
ACTGAGG20100.018.0182194
GGCTCGA29350.017.9458771
GAGGATC16100.017.805153
CTGAGGA14350.017.7764175
AGGAGAG14050.017.7076875
Produced by FastQC (version 0.11.5)