FastQCFastQC Report
Thu 6 Dec 2018
HG77GBGX9_n01_10x_cut_dec18_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG77GBGX9_n01_10x_cut_dec18_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77031981
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGCC702950.019.9080941
TGACGGC304700.019.5260491
CGAGCCG362450.019.4471972
GCATGCG477100.019.402181
CGGACTG383400.019.2652841
GTAAACG571600.019.2429168
CGAGAAG499850.019.2187821
TGAGAGG497850.019.2031691
AGCAACA581550.019.1721573
GTAACTC572950.019.1392333
CTGAAAC380600.019.1310731
TCGAGGC538750.019.0709231
CTCCGTA656350.019.0602657
CGAGGCT438600.019.0512642
GAGGCTC438850.019.0254383
GAGGAGA198050.019.0110914
TGCGAGT598950.018.9761854
CAGTAAC854650.018.9672891
CCCTAAC470600.018.9571279
TCCGTAG605500.018.9459488
Produced by FastQC (version 0.11.5)