FastQCFastQC Report
Thu 6 Dec 2018
HG77GBGX9_n01_10x_cut_dec18_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG77GBGX9_n01_10x_cut_dec18_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59752709
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGCC550000.019.9061011
CGAGCCG283950.019.5020962
TGACGGC239150.019.3923971
CGAGAAG384450.019.2885321
AGCAACA444200.019.287753
GCATGCG366750.019.2715991
GTAAACG437600.019.2303128
CGGACTG291800.019.2225111
TGAGAGG388700.019.1667941
CTCCGTA510050.019.1461727
CTGAAAC296900.019.129311
GTAACTC455200.019.1215133
TCGAGGC404850.019.0403421
CAGTAAC664350.019.0312061
TCCGTAG471350.018.9939868
GAGGAGA154950.018.9661944
TGCGAGT463650.018.949614
CCCTAAC353400.018.9331939
CGAGGCT331800.018.917452
GAGGCTC332000.018.8928343
Produced by FastQC (version 0.11.5)