FastQCFastQC Report
Thu 6 Dec 2018
HG77GBGX9_n01_10x_cut_dec18_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG77GBGX9_n01_10x_cut_dec18_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53674591
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGCC487750.020.0013311
TGACGGC211050.019.541721
CGAGCCG251950.019.4744242
AGCAACA399400.019.3246443
GCATGCG331700.019.2728121
GTAAACG398250.019.2423468
TCGAGGC365250.019.230621
CGGACTG263950.019.1863671
GTAACTC406150.019.1844393
CGAGAAG343700.019.1652181
TGAGAGG343350.019.11441
CTGAAAC267000.019.1088031
CCCTAAC324600.019.0018629
GACGGCA182100.018.9816132
CTCCGTA465950.018.9638427
AGACACC231900.018.9572145
GACCGGA142550.018.945879
CGTAGTA398450.018.92971210
CGAGGCT304250.018.9256212
GTTGTGG259700.018.918698
Produced by FastQC (version 0.11.5)