FastQCFastQC Report
Thu 6 Dec 2018
HG77GBGX9_n01_10x_cut_dec18_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG77GBGX9_n01_10x_cut_dec18_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53881606
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGCC493100.019.9993571
CGAGCCG255650.019.447392
GCATGCG337100.019.3503231
TGACGGC210900.019.331451
CGAGAAG350450.019.2994651
AGCAACA403100.019.2539463
CTGAAAC271450.019.2287921
GTAACTC406200.019.194893
GTAAACG398850.019.171378
CTCCGTA461000.019.1673857
ATGCGAG165800.019.1119633
CGAGGCT296000.019.1020982
TCGAGGC363600.019.0896221
TGAGAGG348700.019.0379121
CGGACTG264900.019.030261
TCCGTAG430650.018.950498
GACCGGA147850.018.9415119
CCCTAAC320350.018.9295449
CAGTAAC596800.018.9281181
CGATTGA277100.018.9201031
Produced by FastQC (version 0.11.5)