FastQCFastQC Report
Thu 6 Dec 2018
HG77GBGX9_n01_10x_ctr2_dec18_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG77GBGX9_n01_10x_ctr2_dec18_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44138178
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC303300.020.070311
ATAACGC283000.019.7100621
GGGCGTG168800.019.2022932
TGGGCGT234100.019.1030371
ACTGCTC308800.018.9850031
TGAACCA201850.018.9393713
CATATTC317350.018.9236011
CACGAGA195850.018.8563828
AGCTGAA218650.018.8014728
GAACCAC170650.018.7902874
TGGCGAG107200.018.7759083
TGAGCCG120450.018.7730751
GTTGCAC210050.018.759734
ACGAGAG197100.018.726169
ATGGCGA107550.018.7154642
GCACGTC215300.018.6746257
CAATGGA150100.018.6504388
TCTGCGA153050.018.4761968
GCAGCGA149100.018.4660916
TTGCACG204800.018.4511345
Produced by FastQC (version 0.11.5)